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FEMS Microbiol Lett. 2012 Dec;337(1):61-72. doi: 10.1111/1574-6968.12010. Epub 2012 Oct 19.

Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica.

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1
Agricultural Research Service, U.S. Department of Agriculture, Athens, GA 30605, USA. jean.guard@ars.usda.gov

Abstract

Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than Kauffmann-White serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S. enterica while maintaining reliability may encourage routine testing and research.

PMID:
22998607
PMCID:
PMC3558799
DOI:
10.1111/1574-6968.12010
[Indexed for MEDLINE]
Free PMC Article
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