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Biochem Biophys Res Commun. 2012 Oct 12;427(1):171-7. doi: 10.1016/j.bbrc.2012.09.036. Epub 2012 Sep 17.

Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells.

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School of Life Sciences and Biotechnology, Korea University, 5ga Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.


Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.

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