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Plant Signal Behav. 2012 Nov;7(11):1428-30. doi: 10.4161/psb.22086. Epub 2012 Sep 18.

Intracellular localization and physiological function of a rice Ca²⁺-permeable channel OsTPC1.

Author information

1
Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, Japan.

Abstract

Two-pore channels (TPCs) are cation channels with a voltage-sensor domain conserved in plants and animals. Rice OsTPC1 is predominantly localized to the plasma membrane (PM), and assumed to play an important role as a Ca²⁺-permeable cation channel in the regulation of cytosolic Ca²⁺ rise and innate immune responses including hypersensitive cell death and phytoalexin biosynthesis in cultured rice cells triggered by a fungal elicitor, xylanase from Trichoderma viride. In contrast, Arabidopsis AtTPC1 is localized to the vacuolar membrane (VM). To gain further insights into the intracellular localization of OsTPC1, we stably expressed OsTPC1-GFP in tobacco BY-2 cells. Confocal imaging and membrane fractionation revealed that, unlike in rice cells, the majority of OsTPC1-GFP fusion protein was targeted to the VM in tobacco BY-2 cells. Intracellular localization and functions of the plant TPC family is discussed.

KEYWORDS:

Ca2+ channel; intracellular localization; plant cells; two-pore channel 1

PMID:
22990444
PMCID:
PMC3548864
DOI:
10.4161/psb.22086
[Indexed for MEDLINE]
Free PMC Article

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