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J Biophotonics. 2013 Aug;6(8):622-30. doi: 10.1002/jbio.201200093. Epub 2012 Sep 14.

Multiphoton imaging of freezing and heating effects in plant leaves.

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JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany.


Thermally-induced changes in Arabidopsis thaliana leaves were investigated with a novel cryo microscope by multiphoton, fluorescence lifetime and spectral imaging as well as micro spectroscopy. Samples were excited with fs pulses in the near-infrared range and cooled/heated in a cryogenic chamber. The results show morphological changes in the chloroplast distribution as well as a shift from chlorophyll to cell-wall fluorescence with decreasing temperature. At temperatures below -40 °C, also second harmonic generation was observed. The measurements illustrate the suitability of multiphoton imaging to investigate thermally-induced changes at temperatures used for cryopreservation as well as for basic investigations of thermal effects on plant tissue in general.


chlorophyll fluorescence; cryomicroscopy; cryopreservation; multiphoton imaging

[Indexed for MEDLINE]

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