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Int J Gynaecol Obstet. 2012 Dec;119(3):234-8. doi: 10.1016/j.ijgo.2012.07.010. Epub 2012 Sep 15.

Syncytiotrophoblast-derived microparticle shedding in early-onset and late-onset severe pre-eclampsia.

Author information

1
Department of Obstetrics and Gynecology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.

Abstract

OBJECTIVE:

To determine the concentration of syncytiotrophoblast-derived microparticles (STBMs) in the maternal circulation and the rate of syncytiotrophoblast apoptosis in the placenta of patients with early-onset and late-onset severe pre-eclampsia.

METHODS:

A prospective case-control study was conducted that enrolled 15 women with early-onset severe pre-eclampsia, 15 women with late-onset severe pre-eclampsia, and 10 women with normal pregnancies. Plasma STBM levels were measured by enzyme-linked immunosorbent assay, while placental levels of active caspase-3 were determined by western blotting. Human umbilical vein endothelial cells (HUVECs) were cultured with STBMs and the proliferation and apoptosis rates of the HUVECs assessed.

RESULTS:

Levels of STBMs in the early-onset group (71.2 ± 20.7 ng/mL) were significantly higher than those detected in the late-onset group (41.9 ± 29.7 ng/mL) and the control group (26.3 ± 11.2 ng/mL) (P<0.05). The amount of active caspase-3 was increased in the early-onset (0.85 ± 0.61) and late-onset groups (0.77 ± 0.46) relative to the control group (0.32 ± 0.15) (P<0.05). Proliferation of HUVECs was inhibited, while apoptosis was elevated, following co-culture with STBMs.

CONCLUSION:

Shedding of STBMs into the maternal circulation occurs in greater amounts in early-onset pre-eclampsia than in late-onset pre-eclampsia.

PMID:
22986096
DOI:
10.1016/j.ijgo.2012.07.010
[Indexed for MEDLINE]

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