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J Appl Microbiol. 2012 Dec;113(6):1530-9. doi: 10.1111/j.1365-2672.2012.05432.x. Epub 2012 Sep 17.

Bacteriophages are synergistic with bacterial interference for the prevention of Pseudomonas aeruginosa biofilm formation on urinary catheters.

Author information

1
Section of Infectious Diseases, Department of Medicine, Baylor College of Medicine, Houston, TX, USA.

Abstract

AIMS:

We hypothesized that pretreating urinary catheters with benign Escherichia coli HU2117 plus an antipseudomonal bacteriophage (ΦE2005-A) would prevent Pseudomonas aeruginosa biofilm formation on catheters--a pivotal event in the pathogenesis of catheter-associated urinary tract infection (CAUTI).

METHODS AND RESULTS:

Silicone catheter segments were exposed to one of four pretreatments (sterile media; E. coli alone; phage alone; E. coli plus phage), inoculated with P. aeruginosa and then incubated up to 72 h in human urine before rinsing and sonicating to recover adherent bacteria. Pseudomonas aeruginosa adherence to catheters was almost 4 log(10) units lower when pretreated with E. coli plus phage compared to no pretreatment (P < 0.001) in 24-h experiments and more than 3 log(10) units lower in 72-h experiments (P < 0.05). Neither E. coli nor phage alone generated significant decreases.

CONCLUSIONS:

The combination of phages with a pre-established biofilm of E. coli HU2117 was synergistic in preventing catheter colonization by P. aeruginosa.

SIGNIFICANCE AND IMPACT OF THE STUDY:

We describe a synergistic protection against colonization of urinary catheters by a common uropathogen. Escherichia coli-coated catheters are in clinical trials; adding phage may offer additional benefit.

PMID:
22985454
PMCID:
PMC3501575
DOI:
10.1111/j.1365-2672.2012.05432.x
[Indexed for MEDLINE]
Free PMC Article

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