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Cytotherapy. 2012 Nov;14(10):1258-75. doi: 10.3109/14653249.2012.715243. Epub 2012 Sep 18.

Immune reconstitution after anti-thymocyte globulin-conditioned hematopoietic cell transplantation.

Author information

1
University of Calgary/Alberta Health Services, Calgary, Alberta, Canada. mbosch@ucalgary.ca

Abstract

BACKGROUND AIMS:

Anti-thymocyte globulin (ATG) is being used increasingly to prevent graft-versus-host disease (GvHD); however, its impact on immune reconstitution is relatively unknown. We (i) studied immune reconstitution after ATG-conditioned hematopoietic cell transplantation (HCT), (ii) determined the factors influencing the reconstitution, and (iii) compared it with non-ATG-conditioned HCT.

METHODS:

Immune cell subset counts were determined at 1-24 months post-transplant in 125 HCT recipients who received ATG during conditioning. Subset counts were also determined in 46 non-ATG-conditioned patients (similarly treated).

RESULTS:

(i) Reconstitution after ATG-conditioned HCT was fast for innate immune cells, intermediate for B cells and CD8 T cells, and very slow for CD4 T cells and invariant natural killer T (iNKT) (iNKT) cells. (ii) Faster reconstitution after ATG-conditioned HCT was associated with a higher number of cells of the same subset transferred with the graft in the case of memory B cells, naive CD4 T cells, naive CD8 T cells, iNKT cells and myeloid dendritic cells; lower recipient age in the case of naive CD4 T cells and naive CD8 T cells; cytomegalovirus recipient seropositivity in the case of memory/effector T cells; an absence of GvHD in the case of naive B cells; lower ATG serum levels in the case of most T-cell subsets, including iNKT cells; and higher ATG levels in the case of NK cells and B cells. (iii) Compared with non-ATG-conditioned HCT, reconstitution after ATG-conditioned HCT was slower for CD4 T cells, and faster for NK cells and B cells.

CONCLUSIONS:

ATG worsens the reconstitution of CD4 T cells but improves the reconstitution of NK and B cells.

PMID:
22985195
PMCID:
PMC3681879
DOI:
10.3109/14653249.2012.715243
[Indexed for MEDLINE]
Free PMC Article

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