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Mol Cell Proteomics. 2012 Dec;11(12):1815-28. doi: 10.1074/mcp.M112.020255. Epub 2012 Sep 14.

Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.

Author information

1
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.

Abstract

Here we describe the function of a previously uncharacterized protein, named family with sequence similarity 60 member A (FAM60A) that maps to chromosome 12p11 in humans. We use quantitative proteomics to determine that the main biochemical partners of FAM60A are subunits of the Sin3 deacetylase complex and show that FAM60A resides in active HDAC complexes. In addition, we conduct gene expression pathway analysis and find that FAM60A regulates expression of genes that encode components of the TGF-beta signaling pathway. Moreover, our studies reveal that loss of FAM60A or another component of the Sin3 complex, SDS3, leads to a change in cell morphology and an increase in cell migration. These studies reveal the function of a previously uncharacterized protein and implicate the Sin3 complex in suppressing cell migration.

PMID:
22984288
PMCID:
PMC3518139
DOI:
10.1074/mcp.M112.020255
[Indexed for MEDLINE]
Free PMC Article

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