Format

Send to

Choose Destination
FEBS Lett. 1990 Jan 29;260(2):198-200.

Inhibition of epidermal growth factor-induced DNA synthesis by tyrosine kinase inhibitors.

Author information

1
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.

Abstract

We prepared methyl 2,5-dihydroxycinnamate as a stable analogue of erbstatin, a tyrosine kinase inhibitor. This analogue was about 4 times more stable than erbstatin in calf serum. It inhibited epidermal growth factor receptor-associated tyrosine kinase in vitro with an IC50 of 0.15 micrograms/ml. It also inhibited in situ autophosphorylation of epidermal growth factor receptor in A431 cells. Methyl 2,5-dihydroxycinnamate was shown to delay the S-phase induction by epidermal growth factor in quiescent normal rat kidney cells, without affecting the total amount of DNA synthesis. The effect of erbstatin on S-phase induction was smaller, possibly because of its shorter life time.

PMID:
2298299
DOI:
10.1016/0014-5793(90)80102-o
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center