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Methods Mol Biol. 2013;919:259-71. doi: 10.1007/978-1-62703-029-8_23.

Cytokine analysis by immunoaffinity capillary electrophoresis.

Author information

1
Micro Analytical Immunochemistry Unit, Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.

Abstract

Immunoaffinity capillary electrophoresis (ICE) is a powerful tool used to detect and quantify target proteins of interest in complex biological fluids. The target analyte is captured and bound to antibodies immobilized onto the wall of a capillary, labeled in situ with a fluorescent dye, eluted and detected online using laser-induced fluorescence following electrophoretic separation. Here, we illustrate how to construct an immunoaffinity capillary and utilize it to run ICE in order to capture and quantify target cytokines and chemokines from a clinical sample.

PMID:
22976107
PMCID:
PMC4167406
DOI:
10.1007/978-1-62703-029-8_23
[Indexed for MEDLINE]
Free PMC Article

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