Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Protoc Cell Biol. 2012 Sep;Chapter 22:Unit22.16. doi: 10.1002/0471143030.cb2216s56.

Assays to measure nuclear mechanics in interphase cells.

Author information

1
Cornell University, Ithaca, New York, USA.

Abstract

The nucleus is the characteristic hallmark of all eukaryotic cells. The physical properties of the nucleus reflect important biological characteristics, such as chromatin organization or nuclear envelope composition; they can also directly affect cellular function, e.g., when cells pass through narrow constrictions, where the stiff nucleus may present a limiting factor. We present two complementary techniques to probe the mechanical properties of the nucleus. In the first, nuclear stiffness relative to the surrounding cytoskeleton is inferred from induced nuclear deformations during strain application to cells on an elastic substrate. In the second approach, nuclear deformability is deduced from the transit time through a perfusion-based microfabricated device with constrictions smaller than the size of the nucleus. These complementary methods, which can be applied to measure nuclear stiffness in large numbers of living adherent or suspended cells, can help identify important changes in nuclear mechanics associated with disease or development.

PMID:
22968843
PMCID:
PMC3605726
DOI:
10.1002/0471143030.cb2216s56
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center