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Talanta. 2012 Sep 15;99:625-30. doi: 10.1016/j.talanta.2012.06.049. Epub 2012 Jun 26.

Detection of DNA damage by using hairpin molecular beacon probes and graphene oxide.

Author information

1
Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Chemistry and Chemical Engineering, Southeast University, Jiangning District, Nanjing 211189, PR China.

Abstract

A hairpin molecular beacon tagged with carboxyfluorescein in combination with graphene oxide as a quencher reagent was used to detect the DNA damage by chemical reagents. The fluorescence of molecular beacon was quenched sharply by graphene oxide; while in the presence of its complementary DNA the quenching efficiency decreased because their hybridization prevented the strong adsorbability of molecular beacon on graphene oxide. If the complementary DNA was damaged by a chemical reagent and could not form intact duplex structure with molecular beacon, more molecular beacon would adsorb on graphene oxide increasing the quenching efficiency. Thus, damaged DNA could be detected based on different quenching efficiencies afforded by damaged and intact complementary DNA. The damage effects of chlorpyrifos-methyl and three metabolites of styrene such as mandelieaeids, phenylglyoxylieaeids and epoxystyrene on DNA were studied as models. The method for detection of DNA damage was reliable, rapid and simple compared to the biological methods.

PMID:
22967603
DOI:
10.1016/j.talanta.2012.06.049
[Indexed for MEDLINE]

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