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Nucleic Acids Res. 2012 Nov 1;40(20):10215-27. doi: 10.1093/nar/gks747. Epub 2012 Sep 10.

Regulation of the nucleosome unwrapping rate controls DNA accessibility.

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1
Department of Physics, The Ohio State University, Columbus, OH 43210, USA.

Abstract

Eukaryotic genomes are repetitively wrapped into nucleosomes that then regulate access of transcription and DNA repair complexes to DNA. The mechanisms that regulate extrinsic protein interactions within nucleosomes are unresolved. We demonstrate that modulation of the nucleosome unwrapping rate regulates protein binding within nucleosomes. Histone H3 acetyl-lysine 56 [H3(K56ac)] and DNA sequence within the nucleosome entry-exit region additively influence nucleosomal DNA accessibility by increasing the unwrapping rate without impacting rewrapping. These combined epigenetic and genetic factors influence transcription factor (TF) occupancy within the nucleosome by at least one order of magnitude and enhance nucleosome disassembly by the DNA mismatch repair complex, hMSH2-hMSH6. Our results combined with the observation that ∼30% of Saccharomyces cerevisiae TF-binding sites reside in the nucleosome entry-exit region suggest that modulation of nucleosome unwrapping is a mechanism for regulating transcription and DNA repair.

PMID:
22965129
PMCID:
PMC3488218
DOI:
10.1093/nar/gks747
[Indexed for MEDLINE]
Free PMC Article
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