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Drug Test Anal. 2012 Sep;4(9):733-8. doi: 10.1002/dta.1402. Epub 2012 Sep 7.

Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential.

Author information

1
Doping Control Laboratory (DoCoLab), Department of Clinical Chemistry, Microbiology and Immunology, Ghent University (UGent), Technologiepark 30, B-9052 Zwijnaarde, Belgium. Simone.Esposito@UGent.be

Abstract

The formulation TB-500 is suspected to be used as doping agent in sport. This work describes the detection and the identification of the N-terminal acetylated 17-23 fragment of human thymosin beta 4 (Ac-LKKTETQ) in TB-500 by means of high-performance liquid chromatography/high resolution mass spectrometry using an Orbitrap Exactive benchtop mass spectrometer. Ac-LKKTETQ was also synthesized by solid-phase peptide synthesis, and an analytical strategy for detection in plasma and urine by high-performance liquid chromatography/low resolution triple-quadrupole mass spectrometry was suggested.

PMID:
22962027
DOI:
10.1002/dta.1402
[Indexed for MEDLINE]

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