Format

Send to

Choose Destination
See comment in PubMed Commons below
Prog Biophys Mol Biol. 2012 Oct-Nov;110(2-3):178-95. doi: 10.1016/j.pbiomolbio.2012.07.012. Epub 2012 Aug 7.

Electrophysiological and contractile function of cardiomyocytes derived from human embryonic stem cells.

Author information

1
Department of Biomedical Engineering, The Johns Hopkins University, 720 Rutland Ave., Baltimore, MD 21205, USA.

Abstract

Human embryonic stem cells have emerged as the prototypical source from which cardiomyocytes can be derived for use in drug discovery and cell therapy. However, such applications require that these cardiomyocytes (hESC-CMs) faithfully recapitulate the physiology of adult cells, especially in relation to their electrophysiological and contractile function. We review what is known about the electrophysiology of hESC-CMs in terms of beating rate, action potential characteristics, ionic currents, and cellular coupling as well as their contractility in terms of calcium cycling and contraction. We also discuss the heterogeneity in cellular phenotypes that arises from variability in cardiac differentiation, maturation, and culture conditions, and summarize present strategies that have been implemented to reduce this heterogeneity. Finally, we present original electrophysiological data from optical maps of hESC-CM clusters.

PMID:
22958937
PMCID:
PMC3910283
DOI:
10.1016/j.pbiomolbio.2012.07.012
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center