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Pharm Biol. 2012 Oct;50(10):1330-6. doi: 10.3109/13880209.2012.675339.

Methanol extract of dried exudate of Commiphora mukul prevents bone resorption in ovariectomized rats.

Author information

1
Department of Pharmacognosy, Manipal College of Pharmaceutical sciences, Manipal University, Madhavnagar, Manipal, India. saleem.khan@manipal.edu

Abstract

CONTEXT:

Gum guggul, a resinous exudate of the plant Commiphora mukul Engl. (Burseraceae), has been found efficacious in the treatment of bone fractures, arthritis, and hyperlipidemic disorders.

OBJECTIVE:

The present study is an effort to explore the anti-bone-resorptive potential of the dried methanol extract of the gummy exudate of C. mukul (MECM) in ovariectomized rat model.

MATERIALS AND METHODS:

The animals were randomly divided into five groups of equal size (n = 6). Animals in all the groups were ovariectomized except group 1, which was sham operated. Groups 3, 4 and 5 were treated with Raloxifene, MECM 250 mg/kg and MECM 500 mg/kg, respectively. The 2nd group was fed with vehicle.

ASSESSMENT:

biochemical estimations, viz., alkaline phosphatase (ALP), tartarate resistant acid phosphatase (TRAP), serum calcium (Ca); biomechanical evaluations, and histopathological examinations.

RESULTS:

The LD(50) of MECM was found to be > 2500 mg/kg orally. A significant elevation was observed in the ALP, TRAP, Ca and cholesterol levels in the 2nd group with a significant reduction in biomechnical strength. Groups 3, 4 and 5, showed a significant reduction in TRAP and ALP levels (p < 0.001). The Ca levels were normalized in the groups 4 and 5, while cholesterol levels dropped in group 5. The bone strength, however, was normalized in all the groups (p < 0.001) along with the histopathology.

DISCUSSION AND CONCLUSION:

Findings suggested a significant gain in bone strength and nearly complete restoration of bone microarchitecture along with lowered levels of TRAP indicating the anti-bone resorptive potential of the extract.

PMID:
22957793
DOI:
10.3109/13880209.2012.675339
[Indexed for MEDLINE]

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