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PLoS One. 2012;7(9):e41990. doi: 10.1371/journal.pone.0041990. Epub 2012 Sep 5.

Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy.

Author information

1
Biological Faculty, Moscow State University, Russia.

Abstract

This paper presents a nonivasive approach to study redox state of reduced cytochromes c, c1 and b of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes c, c1 and b. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes c, c1 and b in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes c, c1 and b in the rod-shaped cardiomyocytes caused by H2O2-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome c in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.

PMID:
22957018
PMCID:
PMC3434226
DOI:
10.1371/journal.pone.0041990
[Indexed for MEDLINE]
Free PMC Article

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