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Food Chem. 2012 Dec 1;135(3):1330-6. doi: 10.1016/j.foodchem.2012.05.107. Epub 2012 Jun 7.

Direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in food samples without derivatisation step by a sensitive and specific monoclonal antibody based ELISA.

Author information

1
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.

Abstract

AMOZ (3-amino-5-methylmorpholino-2-oxazolidinone) is the metabolite of furaltadone (FTD) which has been banned as a veterinary drug. In this study, three AMOZ derivatives (CPAMOZ) were synthesised and monoclonal antibody against o-nitrobenzaldehyde derivatived AMOZ (NPAMOZ) was produced. The IC(50) value of the ELISA for NPAMOZ was 0.17ngml(-1). The cross reactivity values of the assay with NPAMOZ, CPAMOZ, AMOZ and FTA were 100%, 61.7%, 14.8% and 10.4%, respectively. As no NPAMOZ or CPAMOZ exist if no derivitizing reagent added and FTA is quite unstable, therefore, 14.8% of cross reactivity with AMOZ makes the ELISA capable of directly detecting AMOZ without derivatisation. AMOZ spiked food samples were analysed by ELISA. Recoveries of 72.6-121.2% and intra-assay coefficients of variation of 6.1-17.7% were obtained. The proposed ELISA which also confirmed by HPLC with a good correlation provides an alternative sensitive and fast quantitative method for directly detecting AMOZ in food samples.

PMID:
22953862
DOI:
10.1016/j.foodchem.2012.05.107
[Indexed for MEDLINE]

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