Format

Send to

Choose Destination
See comment in PubMed Commons below
Ann Lab Med. 2012 Sep;32(5):359-61. doi: 10.3343/alm.2012.32.5.359. Epub 2012 Aug 13.

Multiplex PCR for rapid detection of genes encoding class A carbapenemases.

Author information

1
Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea.

Abstract

In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-β-lactamase nonproducers.

KEYWORDS:

Carbapenemase; GES; KPC; Multiplex PCR

PMID:
22950072
PMCID:
PMC3427824
DOI:
10.3343/alm.2012.32.5.359
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for The Korean Society for Laboratory Medicine Icon for PubMed Central
    Loading ...
    Support Center