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Int J Mol Sci. 2012;13(8):10268-95. doi: 10.3390/ijms130810268. Epub 2012 Aug 17.

Biogenesis and mechanism of action of small non-coding RNAs: insights from the point of view of structural biology.

Author information

1
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, Lisboa 1649-028, Portugal; E-Mail: marinacosta@fm.ul.pt.

Abstract

Non-coding RNAs are dominant in the genomic output of the higher organisms being not simply occasional transcripts with idiosyncratic functions, but constituting an extensive regulatory network. Among all the species of non-coding RNAs, small non-coding RNAs (miRNAs, siRNAs and piRNAs) have been shown to be in the core of the regulatory machinery of all the genomic output in eukaryotic cells. Small non-coding RNAs are produced by several pathways containing specialized enzymes that process RNA transcripts. The mechanism of action of these molecules is also ensured by a group of effector proteins that are commonly engaged within high molecular weight protein-RNA complexes. In the last decade, the contribution of structural biology has been essential to the dissection of the molecular mechanisms involved in the biosynthesis and function of small non-coding RNAs.

KEYWORDS:

DGCR8; Dicer; X-ray crystallography; argonaute; miRNAs; microprocessor; non-coding RNA; structural biology

PMID:
22949860
PMCID:
PMC3431858
DOI:
10.3390/ijms130810268
[Indexed for MEDLINE]
Free PMC Article
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