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Int J Mol Sci. 2012;13(7):9194-206. doi: 10.3390/ijms13079194. Epub 2012 Jul 23.

Antioxidative properties of crude polysaccharides from Inonotus obliquus.

Author information

1
College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China; E-Mails: mhb1025@sina.com (H.M.); wmyzyqyybfk@126.com (A.Z.); wuxia200758@163.com (W.Z.); moohybo@yahoo.cn (G.C.).

Abstract

The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS) including 1,1'-Diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H(2)O(2)-induced PC12 cell death were investigated. Results showed that I. obliquus polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20μg/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from I. obliquus might contribute to diverse medicinal and nutritional values of this mushroom.

KEYWORDS:

Inonotus obliquus; antioxidant; polysaccharide; reactive oxygen species

PMID:
22942760
PMCID:
PMC3430291
DOI:
10.3390/ijms13079194
[Indexed for MEDLINE]
Free PMC Article

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