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Int J Oncol. 2012 Nov;41(5):1653-61. doi: 10.3892/ijo.2012.1614. Epub 2012 Aug 30.

miR-203 inhibits the migration and invasion of esophageal squamous cell carcinoma by regulating LASP1.

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1
Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chiba, Japan.

Abstract

The expression of microRNA-203 (miR-203) in esophageal squamous cell carcinoma (ESCC) tissues is remarkably lower than that in non‑ESCC tissues. We investigated how miR-203 could influence the development of ESCC cells. Our analyses revealed that miR-203 inhibited the migration and invasion of ESCC cells. Genome-wide gene expression data and target site inhibition assays showed that miR-203 appears to directly regulate LIM and SH3 protein 1 (LASP1). The knockdown of LASP1 resulted in inhibition of the migration and invasion of ESCC cells. Our results suggest that miR-203 and its target LASP1, may be associated with the progression of ESCC. In clinical ESCC specimens, the expression levels of miR-203, which were lower compared to those in normal tissues, were inversely correlated with the mRNA expression levels of LASP1. Moreover, we found that there was a significant correlation between the expression levels of miR-203 and the relapse‑free survival. The identification of a cancer network regulated by miR-203 could provide new insights into the potential mechanisms of the progression of ESCC.

PMID:
22940702
DOI:
10.3892/ijo.2012.1614
[Indexed for MEDLINE]

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