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J Clin Microbiol. 2012 Nov;50(11):3751-3. doi: 10.1128/JCM.01670-12. Epub 2012 Aug 29.

Rapid determination of lymphogranuloma venereum serovars of Chlamydia trachomatis by quantitative high-resolution melt analysis (HRMA).

Author information

1
Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia. jimmy.twin@mcri.edu.au

Abstract

A quantitative high-resolution melt analysis assay was developed to differentiate lymphogranuloma venereum-causing serovars of Chlamydia trachomatis (L1 to L3) from other C. trachomatis serovars (D to K). The detection limit of this assay is approximately 10 copies per reaction, comparable to the limits of other quantitative-PCR-based methods.

PMID:
22933594
PMCID:
PMC3486269
DOI:
10.1128/JCM.01670-12
[Indexed for MEDLINE]
Free PMC Article

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