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ISME J. 2013 Feb;7(2):237-43. doi: 10.1038/ismej.2012.94. Epub 2012 Aug 30.

Sizing up metatranscriptomics.

Author information

1
Department of Marine Sciences, University of Georgia, Athens, GA 30602-3636, USA. mmoran@uga.edu

Abstract

A typical marine bacterial cell in coastal seawater contains only ∼200 molecules of mRNA, each of which lasts only a few minutes before being degraded. Such a surprisingly small and dynamic cellular mRNA reservoir has important implications for understanding the bacterium's responses to environmental signals, as well as for our ability to measure those responses. In this perspective, we review the available data on transcript dynamics in environmental bacteria, and then consider the consequences of a small and transient mRNA inventory for functional metagenomic studies of microbial communities.

PMID:
22931831
PMCID:
PMC3554401
DOI:
10.1038/ismej.2012.94
[Indexed for MEDLINE]
Free PMC Article

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