Send to

Choose Destination
See comment in PubMed Commons below
Cell Mol Life Sci. 2013 Apr;70(7):1185-206. doi: 10.1007/s00018-012-1120-4. Epub 2012 Aug 28.

Recent progress in intein research: from mechanism to directed evolution and applications.

Author information

  • 1Institute of Biochemistry, University of Münster, Wilhelm-Klemm-Str. 2, 48149, Münster, Germany.


Inteins catalyze a post-translational modification known as protein splicing, where the intein removes itself from a precursor protein and concomitantly ligates the flanking protein sequences with a peptide bond. Over the past two decades, inteins have risen from a peculiarity to a rich source of applications in biotechnology, biomedicine, and protein chemistry. In this review, we focus on developments of intein-related research spanning the last 5 years, including the three different splicing mechanisms and their molecular underpinnings, the directed evolution of inteins towards improved splicing in exogenous protein contexts, as well as novel applications of inteins for cell biology and protein engineering, which were made possible by a clearer understanding of the protein splicing mechanism.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center