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Anal Bioanal Chem. 2012 Oct;404(6-7):1631-5. doi: 10.1007/s00216-012-6324-9. Epub 2012 Aug 29.

Novel method to investigate protein carbonylation by iTRAQ strategy.

Author information

1
Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Naples, Italy.

Abstract

This paper reports a novel methodology for relative quantitative analysis of carbonylation sites in proteins by exploiting a new isobaric tag for relative and absolute quantitation (iTRAQ) derivative, iTRAQ hydrazide (iTRAQH), and the analytical power of linear ion trap instruments (QqLIT). Because of its operational simplicity, avoiding time-consuming enrichment procedures, this new strategy seems to be well suited for quantitative large-scale proteomic profiling of carbonylation.

PMID:
22926130
DOI:
10.1007/s00216-012-6324-9
[Indexed for MEDLINE]

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