Format

Send to

Choose Destination
Nat Immunol. 2012 Oct;13(10):1010-9. doi: 10.1038/ni.2402. Epub 2012 Aug 26.

Transcription factor Foxp3 and its protein partners form a complex regulatory network.

Author information

1
Howard Hughes Medical Institute and Immunology Program, Sloan-Kettering Institute, New York, New York, USA.

Abstract

The transcription factor Foxp3 is indispensible for the differentiation and function of regulatory T cells (T(reg) cells). To gain insights into the molecular mechanisms of Foxp3-mediated gene expression, we purified Foxp3 complexes and explored their composition. Biochemical and mass-spectrometric analyses revealed that Foxp3 forms multiprotein complexes of 400-800 kDa or larger and identified 361 associated proteins, ∼30% of which were transcription related. Foxp3 directly regulated expression of a large proportion of the genes encoding its cofactors. Some transcription factor partners of Foxp3 facilitated its expression. Functional analysis of the cooperation of Foxp3 with one such partner, GATA-3, provided additional evidence for a network of transcriptional regulation afforded by Foxp3 and its associates to control distinct aspects of T(reg) cell biology.

PMID:
22922362
PMCID:
PMC3448012
DOI:
10.1038/ni.2402
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center