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Bioengineered. 2013 Jan-Feb;4(1):9-14. doi: 10.4161/bioe.21950. Epub 2012 Aug 24.

Engineering influenza viral vectors.

Author information

1
Center of Excellence for Infectious Diseases, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX, USA.

Abstract

The influenza virus is a respiratory pathogen with a negative-sense, segmented RNA genome. Construction of recombinant influenza viruses in the laboratory was reported starting in the 1980s. Within a short period of time, pioneer researchers had devised methods that made it possible to construct influenza viral vectors from cDNA plasmid systems. Herein, we discuss the evolution of influenza virus reverse genetics, from helper virus-dependent systems, to helper virus-independent 17-plasmid systems, and all the way to 3- and 1- plasmid systems. Successes in the modification of different gene segments for various applications, including vaccine and gene therapies are highlighted.

KEYWORDS:

gene delivery; gene therapy; influenza virus; microRNA delivery; pseudo-typed virus; reverse genetics; vaccine; viral vector

PMID:
22922205
PMCID:
PMC3566024
DOI:
10.4161/bioe.21950
[Indexed for MEDLINE]
Free PMC Article

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