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J Microbiol Methods. 2012 Oct;91(1):144-6. doi: 10.1016/j.mimet.2012.08.006. Epub 2012 Aug 19.

A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures.

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1
Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.

Abstract

A robust, efficient and cost-effective agar that utilises lactose free milk powder for identification of bacterial protease activity in pure cultures and metagenomic screens has been developed and tested on protease positive bacteria, selected strains and false protease positives isolated from a previously constructed metagenomic library.

PMID:
22921428
DOI:
10.1016/j.mimet.2012.08.006
[Indexed for MEDLINE]
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