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J Chromatogr A. 2012 Sep 28;1257:125-30. doi: 10.1016/j.chroma.2012.07.096. Epub 2012 Aug 7.

Capillary electrophoresis as a tool for the characterization of pentosan nanoparticles.

Author information

1
Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. hanin.abdelhaq@iss.it

Abstract

Because capillary zone electrophoresis (CZE) showed higher resolution for highly charged large carbohydrates and complex structures when compared to other chromatographic separation methods, it was chosen for the characterization of nanoparticles (NPs) of pentosan polysulfate (PPS). Thus, using the CZE technique, we developed a reliable, sensitive and rapid protocol that allowed the detection and characterization of PPS NPs. This protocol was able to determine the profile of both the NPs and the species of PPS entrapped into them, and to quantify free and bound PPS showing high reproducibility, acceptable accuracy and a good degree of precision. Moreover, it allowed the evaluation of the size and charge of the NPs. This protocol might be suitable for the characterization of other kinds of NPs also.

PMID:
22921360
DOI:
10.1016/j.chroma.2012.07.096
[Indexed for MEDLINE]

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