Format

Send to

Choose Destination
Fungal Genet Biol. 2012 Oct;49(10):772-8. doi: 10.1016/j.fgb.2012.08.003. Epub 2012 Aug 17.

Tools for high efficiency genetic manipulation of the human pathogen Penicillium marneffei.

Author information

1
Department of Genetics, The University of Melbourne, Parkville, Victoria 3010, Australia.

Abstract

Penicillium marneffei is an opportunistic pathogen of humans and displays a temperature dependent dimorphic transition. Like many fungi, exogenous DNA introduced by DNA mediated transformation is integrated randomly into the genome resulting in inefficient gene deletion and position-specific effects. To enhance successful gene targeting, the consequences of perturbing components of the non-homologous end joining recombination pathway have been examined. The deletion of the KU70 and LIG4 orthologs, pkuA and ligD, respectively, dramatically enhanced the observed homologous recombination frequency leading to efficient gene deletion. While ΔpkuA was associated with reduced genetic stability over-time, ΔligD represents a suitable recipient strain for downstream applications and combined with a modified Gateway™ system for the rapid generation of gene deletion constructs, this represents an efficient pipeline for characterizing gene function in P. marneffei.

PMID:
22921264
DOI:
10.1016/j.fgb.2012.08.003
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center