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J Microbiol Immunol Infect. 2013 Oct;46(5):366-73. doi: 10.1016/j.jmii.2012.07.010. Epub 2012 Aug 21.

Phosphoproteome profiling of the sexually transmitted pathogen Trichomonas vaginalis.

Author information

1
Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan.

Abstract

BACKGROUND/PURPOSE(S):

Trichomoniasis caused by Trichomonas vaginalis is the most common non-viral sexually transmitted infection. Morphological transformation from the trophozoite stage to the amoeboid or pseudocyst stage is crucial for T. vaginalis infection and survival. Protein phosphorylation is a key post-translational modification involved in the regulation of several biological processes in various prokaryotes and eukaryotes. More than 880 protein kinases have been identified in the T. vaginalis genome. However, little is known about the phosphorylation of specific proteins and the distribution of phosphorylated proteins in different stages of the morphological transformation of T. vaginalis.

METHODS:

To obtain a more comprehensive understanding of the T. vaginalis phosphoproteome, we analyzed phosphorylated proteins in the three morphological stages using titanium dioxide combined with LC-MS/MS.

RESULTS:

A total of 93 phosphopeptides originating from 82 unique proteins were identified. Among these proteins, 21 were detected in all stages, 29 were identified in two different stages, and 32 were stage specific.

CONCLUSION:

Identification of stage-specific phosphorylated proteins indicates that phosphorylation of these proteins may play a key role in the morphological transformation of T. vaginalis.

KEYWORDS:

Phosphoproteome; Titanium dioxide enrichment; Trichomonas vaginalis

PMID:
22921107
DOI:
10.1016/j.jmii.2012.07.010
[Indexed for MEDLINE]
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