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Org Biomol Chem. 2012 Oct 14;10(38):7820-5. doi: 10.1039/c2ob25883h.

Quick regulation of mRNA functions by a few seconds of photoirradiation.

Author information

1
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.

Abstract

3-Cyanovinylcarbazole nucleoside, which effectively photocrosslinks to the pyrimidine base in complementary RNA strands, was incorporated into antisense oligonucleotides, and we evaluated the photoreactivity and the sequence selectivity to mutated K-ras oligoRNAs, as well as the regulation of the function of K-ras mRNA. We demonstrated that the reverse transcription and the translation activity of K-ras mRNA were quickly suppressed by a few seconds of photoirradiation with the addition of the photoresponsive antisense ODN.

PMID:
22918386
DOI:
10.1039/c2ob25883h
[Indexed for MEDLINE]

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