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J Hazard Mater. 2013 Nov 15;262:1123-31. doi: 10.1016/j.jhazmat.2012.06.049. Epub 2012 Jul 1.

Arsenate and arsenite exposure modulate antioxidants and amino acids in contrasting arsenic accumulating rice (Oryza sativa L.) genotypes.

Author information

1
Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Lucknow 226001, India; Amity Institute of Environmental Sciences, Amity University, Noida, India.

Abstract

Carcinogenic arsenic (As) concentrations are found in rice due to irrigation with contaminated groundwater in South-East Asia. The present study evaluates comparative antioxidant property and specific amino acid accumulation in contrasting rice genotypes corresponding to differential As accumulation during arsenate (As(V)) and arsenite (As(III)) exposures. The study was conducted on two contrasting As accumulating rice genotypes selected from 303 genotype accessions, in hydroponic conditions. Maximum As accumulation was up to 1181 μg g(-1) dw in the roots of high As accumulating genotype (HARG), and 89 μg g(-1) dw in low As accumulating genotype (LARG) under As(III) exposures. The inorganic As was correlated more significantly upon exposures to As(III) than As(V). In the presence of As(V) various antioxidant enzymes guiacol peroxidase (GPX), ascorbate peroxidase (APX) and superoxide dismutase (SOD) were highly stimulated in HARG. The stress responsive amino acids proline, cysteine, glycine, glutamic acid and methionine showed higher accumulation in HARG than LARG. A clear correlation was found between stress responsive amino acids, As accumulation and antioxidative response. The comparisons between the contrasting genotypes helped to determine the significance of antioxidants and specific amino acid response to As stress.

KEYWORDS:

Amino acids; Antioxidants; Arsenic accumulation; Oryza sativa

PMID:
22917495
DOI:
10.1016/j.jhazmat.2012.06.049
[Indexed for MEDLINE]

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