Format

Send to

Choose Destination
See comment in PubMed Commons below
Mol Endocrinol. 2012 Oct;26(10):1783-92. Epub 2012 Aug 21.

Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome.

Author information

1
513 Parnassus Avenue, Box 0534, San Francisco, California 94143-0534, USA.

Abstract

The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome.

PMID:
22915829
PMCID:
PMC3458219
DOI:
10.1210/me.2012-1176
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center