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[Investigation on blood-sucking habit of Anopheles (Diptera:Culicidae) using multiplex polymerase chain reaction in malaria-endemic area of Chayu County, Tibet].

[Article in Chinese]

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  • 1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.



To determine the blood-sucking habit of anopheline by mosquitoes bloodmeal identification in malaria endemic area Chayu County, Tibet Autonomous Region.


Three villages with different bio-environments were selected as the investigation spots. Light traps were set up outdoor and in livestock sheds from sunset (20:00) to sunrise (8:00) in 3-4 consecutive nights to collect mosquitoes. The trapped anophelines were counted and identified according to morphological criteria and multiple PCR method. A PCR-based methodology according to the mtDNA-cytb variations was used in different mammal hosts to identify bloodmeal sources in engorged mosquitoes. The human blood index (HBI) was assessed to determine the range of hosts.


Among 1 442 anopheline mosquitoes collected by 108 light traps on 13 nights. 1436 (99.6%) belonged to Anopheles maculatus complex, with 85.5% An. pseudo-willmori and 14.5% An. willmori. Positive bloodmeal identification was found from 168 (83%) of 202 field-collected engorged mosquitoes. The crude HBI of An. pseudowillmori and An. willmori were 035 and 0.29, respectively.


An. pseudowillmori and An willmori are both zoophilic and anthropophilic, and An. pseudowillmori shows a higher HBI.

[PubMed - indexed for MEDLINE]
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