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Proteomics. 2012 Aug;12(15-16):2487-92. doi: 10.1002/pmic.201100354.

The OASIS® HLB μElution plate as a one-step platform for manual high-throughput in-gel digestion of proteins and peptide desalting.

Author information

1
Max-Planck-Institute for Biology of Ageing, Bio-MS Facility, Cologne, Germany. franz@age.mpg.de

Abstract

Separation or prefractionation of proteins by gel electrophoresis, followed by in-gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost-effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not free of error if many samples are processed at the same time. The OASIS® HLB μElution plate allows the handling of 96 samples for protein in-gel digest and peptide desalting in a semiautomatic way. Using multichannel pipettes, solutions are added quickly, and with the use of the positive pressure-96 stand all solutions are removed simultaneously. In this article, a complete and detailed protocol for the use of the OASIS® HLB μElution plate is introduced and its effectiveness is shown with 2D- and 1D-gel samples.

PMID:
22903840
DOI:
10.1002/pmic.201100354
[Indexed for MEDLINE]

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