(a) Overview of the experimental design for the deletion of Ppard. KSL, c-KitposSca-1posLinneg, MOI, multiplicity of infection, CAFC, cobblestone area forming cells, LTC-IC, long-term culture initiating cells. (b) Homing capacity to the bone marrow (BM) of indicated HSCs. The mean percentages ± S.D. of donor-derived CD34negKSL cells are shown (n = 3). (c,d) Haematopoietic reconstitution capacity of Ppard-deleted KSL cells in BMT. Recipient mice were transplanted with 1.5x103 PpardΔ/Δ KSL cells plus 2.0x105 competitor cells in competition assays. Results represent the mean percentages ± s.d. of donor-derived cells (n = 4) (c) and multi-lineage haematopoietic contribution (myeloid cells (left), B cells (middle) and T cells (right)) 5 months after transplantation (d). (e,f), Limiting-dilution competitive repopulation analysis in second (e) and third (f) BMT with Ppard-ablated HSCs. (g), Cell cycle status of Ppardlox/lox CD150posCD48negKSL cells by Pyronin Y staining 6 weeks after BMT (n = 4). All error bars indicate s.d.