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Cancer Lett. 2013 Jun 28;334(1):118-26. doi: 10.1016/j.canlet.2012.08.003. Epub 2012 Aug 16.

Grape seed proanthocyanidins inhibit migration potential of pancreatic cancer cells by promoting mesenchymal-to-epithelial transition and targeting NF-κB.

Author information

1
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA.
2
Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA; Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL, USA; Nutrition Obesity Research Center, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA; Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA; Birmingham Veterans Affairs Medical Center, Birmingham, AL, USA. Electronic address: skatiyar@uab.edu.

Abstract

Here we explore the effect of grape seed proanthocyanidins (GSPs) on pancreatic cancer cell migration and the molecular mechanisms underlying these effects. Treatment of human pancreatic cancer cell lines Miapaca-2, PANC-1 and AsPC-1 with GSPs resulted in inhibition of cell migration (19-82%, P<0.01-0.001), which was associated with decreased phosphorylation of ERK1/2 and inactivation of NF-κB. Treatment of cells with UO126, an inhibitor of MEK, and caffeic acid phenethyl ester, an inhibitor of NF-κB, also inhibited the migration of cells (40-80%, P<0.01-0.001). Inhibition of cell migration by GSPs was associated with reversal of the epithelial-to-mesenchymal transition. This was associated with upregulation of E-cadherin and desmoglein-2 and down-regulation of fibronectin, N-cadherin and vimentin.

PMID:
22902508
DOI:
10.1016/j.canlet.2012.08.003
[Indexed for MEDLINE]

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