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PLoS One. 2012;7(8):e42882. doi: 10.1371/journal.pone.0042882. Epub 2012 Aug 10.

Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.

Author information

1
Genomic Health Inc., Redwood City, California, United States of America. jmorlan@genomichealth.com

Abstract

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.

PMID:
22900061
PMCID:
PMC3416766
DOI:
10.1371/journal.pone.0042882
[Indexed for MEDLINE]
Free PMC Article

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