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Hum Vaccin Immunother. 2012 Oct;8(10):1450-3. doi: 10.4161/hv.21283. Epub 2012 Aug 16.

Paracoccidioidomycosis vaccine.

Author information

1
Cell Biology Division, Department of Microbiology, Federal University of São Paulo, São Paulo, Brazil. travassos@unifesp.br

Abstract

Paracoccidioidomycosis is a granulomatous pulmonary infection that is generally controlled by chemotherapy. The efficacy of treatment, however, is limited by the status of the host immune response. The inhibition of a Th-2 immunity or the stimulation of Th-1 cytokines generally increases the efficacy of antifungal drugs. ( 1) This has been achieved by immunization with an internal peptide of the major diagnostic antigen gp43 of Paracoccidioides brasiliensis. Peptide 10 (QTLIAIHTLAIRYAN) elicits an IFN-γ rich Th-1 immune response that protects against experimental intratracheal infection by this fungus. The combination of chemotherapy with P10 immunization showed additive protective effect even after 30 d of infection or in anergic mice, rendering in general, increased production of IL-12 and IFN-γ and reduction of IL-4 and IL-10. Immunotherapy with P10 even in the absence of simultaneous chemotherapy has been effective using various protocols, adjuvants, nanoparticles, P10-primed dendritic cells, and especially a combination of plasmids encoding the P10 minigene and IL-12. Gene therapy, in a long-term infection protocol succeeded in the virtual elimination of the fungus, preserving the lung structure, free from immunopathological side effects.

KEYWORDS:

DNA therapy; Gp43; Paracoccidiodes brasiliensis; Peptide P10; Th-1 immune response; adjuvant; vaccine

PMID:
22894948
PMCID:
PMC3660765
DOI:
10.4161/hv.21283
[Indexed for MEDLINE]
Free PMC Article

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