Format

Send to

Choose Destination
Mol Syst Biol. 2012;8:602. doi: 10.1038/msb.2012.34.

Quantitative measurement of allele-specific protein expression in a diploid yeast hybrid by LC-MS.

Author information

1
Department of Computer Science, Princeton University, Princeton, NJ 08544, USA. zia@uchicago.edu

Abstract

Understanding the genetic basis of gene regulatory variation is a key goal of evolutionary and medical genetics. Regulatory variation can act in an allele-specific manner (cis-acting) or it can affect both alleles of a gene (trans-acting). Differential allele-specific expression (ASE), in which the expression of one allele differs from another in a diploid, implies the presence of cis-acting regulatory variation. While microarrays and high-throughput sequencing have enabled genome-wide measurements of transcriptional ASE, methods for measurement of protein ASE (pASE) have lagged far behind. We describe a flexible, accurate, and scalable strategy for measurement of pASE by liquid chromatography-coupled mass spectrometry (LC-MS). We apply this approach to a hybrid between the yeast species Saccharomyces cerevisiae and Saccharomyces bayanus. Our results provide the first analysis of the relative contribution of cis-acting and trans-acting regulatory differences to protein expression divergence between yeast species.

PMID:
22893000
PMCID:
PMC3435501
DOI:
10.1038/msb.2012.34
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center