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Mol Biosyst. 2012 Nov;8(11):2850-6. doi: 10.1039/c2mb25268f. Epub 2012 Aug 14.

Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy.

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1
Department of Medicine, University of Washington, Seattle, WA 98195, USA. shengp@medicine.washington.edu

Abstract

Disease-associated aberrant glycosylation may be protein specific and glycosylation site specific. Quantitative assessment of glycosylation changes at a site-specific molecular level may represent one of the initial steps for systematically revealing the glycosylation abnormalities associated with a disease or biological state. Comparative quantitative profiling of glycoproteome to provide accurate quantification of site-specific glycosylation occupancy has been a challenging task, requiring a concerted approach drawing from a variety of techniques. In this report, we present a quantitative glycoproteomics method that allows global scale identification and comparative quantification of glycosylation site occupancy using mass spectrometry. We further demonstrated this approach by quantitatively characterizing the N-glycoproteome of human pancreas.

PMID:
22892896
PMCID:
PMC3463725
DOI:
10.1039/c2mb25268f
[Indexed for MEDLINE]
Free PMC Article
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