Format

Send to

Choose Destination
Nat Rev Microbiol. 2012 Sep;10(9):618-30. doi: 10.1038/nrmicro2852.

Dual RNA-seq of pathogen and host.

Author information

1
Institute for Molecular Infection Biology, University of W├╝rzburg, D-97080, Germany.

Abstract

A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.

PMID:
22890146
DOI:
10.1038/nrmicro2852
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center