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Nat Rev Microbiol. 2012 Sep;10(9):618-30. doi: 10.1038/nrmicro2852.

Dual RNA-seq of pathogen and host.

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Institute for Molecular Infection Biology, University of W├╝rzburg, D-97080, Germany.


A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.

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