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New Biol. 1990 May;2(5):441-9.

Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.

Author information

1
E.I. DuPont de Nemours & Co., Inc., Central Research and Development Department, Wilmington, DE 19880-0328.

Abstract

Cre is a 38-kD protein from bacteriophage P1 that catalyzes site-specific recombination between 34-bp loxP sequences. Our previous work has shown that Cre can perform site-specific excisive recombination not only in prokaryotes, but also in eukaryotes such as yeast and cultured mammalian cells. In this work we show that intermolecular Cre-mediated recombination can specifically direct the integration of a loxP-containing circular DNA into a chromosomal loxP site, both in yeast and in mammalian cells. The resulting integrants are predominantly simple single-copy insertions. Cre-mediated recombination thus provides a simple way to direct single-copy site-specific integration of exogenous DNA into the eukaryotic genome.

PMID:
2288914
[Indexed for MEDLINE]

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