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Appl Environ Microbiol. 2012 Oct;78(20):7480-2. Epub 2012 Aug 10.

Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes.

Author information

1
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Abstract

A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46 Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.

PMID:
22885744
PMCID:
PMC3457111
DOI:
10.1128/AEM.01703-12
[Indexed for MEDLINE]
Free PMC Article

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