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Anal Chem. 2012 Sep 4;84(17):7487-94. doi: 10.1021/ac301589z. Epub 2012 Aug 20.

Energy-dependent electron activated dissociation of metal-adducted permethylated oligosaccharides.

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1
Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts 02118-2646, United States.

Abstract

The effects of varying the electron energy and cationizing agents on electron activated dissociation (ExD) of metal-adducted oligosaccharides were explored, using permethylated maltoheptaose as the model system. Across the examined range of electron energy, the metal-adducted oligosaccharide exhibited several fragmentation processes, including electron capture dissociation (ECD) at low energies, hot-ECD at intermediate energies, and electronic excitation dissociation (EED) at high energies. The dissociation threshold depended on the metal charge carrier(s), whereas the types and sequence spans of product ions were influenced by the metal-oligosaccharide binding pattern. Theoretical modeling contributed insight into the metal-dependent behavior of carbohydrates during low-energy ECD. When ExD was applied to a permethylated high mannose N-linked glycan, EED provided more structural information than either collision-induced dissociation (CID) or low-energy ECD, thus demonstrating its potential for oligosaccharide linkage analysis.

PMID:
22881449
PMCID:
PMC3568493
DOI:
10.1021/ac301589z
[Indexed for MEDLINE]
Free PMC Article

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