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EMBO Rep. 2012 Oct;13(10):923-9. doi: 10.1038/embor.2012.119. Epub 2012 Aug 10.

R-loops cause replication impairment and genome instability during meiosis.

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1
Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla-CSIC, Sevilla, Spain.

Abstract

R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.

PMID:
22878416
PMCID:
PMC3463965
DOI:
10.1038/embor.2012.119
[Indexed for MEDLINE]
Free PMC Article
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