Format

Send to

Choose Destination
Stem Cell Res. 2012 Nov;9(3):192-5. doi: 10.1016/j.scr.2012.06.008. Epub 2012 Jul 11.

High-throughput karyotyping of human pluripotent stem cells.

Author information

1
Molecular and Systems Immunology and Stem Cell Biology, Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland. riikka.lund@btk.fi

Abstract

Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis can be performed from low amounts of DNA isolated from whole cell pools with simple data analysis interface. The method enables routine screening of stem cell lines in a cost-efficient high-throughput manner.

PMID:
22877823
PMCID:
PMC3502865
DOI:
10.1016/j.scr.2012.06.008
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center