Format

Send to

Choose Destination
Virology. 2012 Nov 10;433(1):64-72. doi: 10.1016/j.virol.2012.07.015. Epub 2012 Aug 5.

Human cytomegalovirus infection of THP-1 derived macrophages reveals strain-specific regulation of actin dynamics.

Author information

1
Texas A&M Health Science Center, College of Medicine, Department of Microbial and Molecular Pathogenesis, College Station, TX 77843, USA. VSanchez@medicine.tamhsc.edu

Abstract

Human cytomegalovirus (HCMV) remains latent in cells of the myeloid lineage after primary infection. The THP-1 monocytic cell line is conditionally permissive for infection and has been used primarily to study the process of HCMV reactivation when the cells are induced to differentiate. In the present report, we characterized lytic infection in THP-1 derived macrophages using two strains of HCMV, Towne and BAC-derived TR. Our findings indicate that these cells express viral genes of all three kinetic classes and produce extracellular virus, but that there is a delay in these processes relative to productively infected fibroblasts. Importantly, our studies in THP-1 derived macrophages revealed strain-specific differences in pp65 trafficking and actin dynamics. Based on these observations, our studies indicate that differentiated THP-1 cells can serve as a valuable model for lytic infection.

PMID:
22874068
DOI:
10.1016/j.virol.2012.07.015
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center